Extended Data Fig. 6: Endogenous fructose has no impact on cell proliferation.
From: Dietary fructose enhances tumour growth indirectly via interorgan lipid transfer
(a) Western blot analysis of sorbitol dehydrogenase from the indicated cell lines after transfection with either sorbitol dehydrogenase siRNA (+) or scrambled control (-). (b-f) Relative cell number after administering sorbitol dehydrogenase siRNA (SORD siRNA, n = 3-4), scrambled control siRNA (scrambled control, n = 3-5), or sorbitol dehydrogenase siRNA and 10 mM fructose (SORD siRNA + 10 mM fructose, n = 3-4) to the indicated cell lines for 4 days. (g) Analysis of 15 µM solutions of glucose 6-phosphate, fructose 6-phosphate, glucose 1-phosphate, and fructose 1-phosphate with a HILICpak VT 50-2D column. Each is chromatographically resolved from one another. Solutions were prepared by using authentic standards. (h-m) Isotopologue distribution for fructose 1-phosphate after labelling with [U-13C] fructose for 4 h (n = 3). Labelled fructose 1-phosphate indicates KHK activity. All experiments in this figure were performed once. (b-f) P values determined by ordinary one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and n.s. is not significant. All data shown as mean ± standard deviation.
