Extended Data Fig. 11: Dietary fructose increases the availability of circulating lipids for the tumour.
From: Dietary fructose enhances tumour growth indirectly via interorgan lipid transfer
(a) Top 5 significant serum lipids from ANOVA. Data were collected from C57 BL/6 mice on control diets supplemented with normal water (n = 4), 10% glucose water (Glc water, n = 4), 10% fructose water (Frc water, n = 5), or 20% HFCS (HFCS water, n = 6) for six weeks. Red indicates an increase in relative serum concentration. Blue indicates a decrease in relative serum concentration. (b) Total labelling for serum LPC 18:1 after providing C57 BL/6 mice (n = 5) access to 5% [U-13C] fructose water for 60 h. Data were corrected for natural 13C abundance. (c) Schematic of labelling experiment for fed and fasted mice. Mice were given access to 10% [U-13C] fructose water for 84 h. (d) Fractional carbon atom labelling for serum 16:0 acylcarnitine and serum LPC 18:2 after administration of 10% [U-13C] fructose in the drinking water. Fed state was t = 84 h and fasting state was t = 96 h according to schematic in (c). Acylcarnitines provided an estimate of fatty acid labelling and LPC 18:2 an estimate of glycerol labelling. (e) Quantitative comparison of LPC 18:1 levels in the serum to matched tumour interstitial fluid (TIF) from multiple tumour types (TC-1, n = 4; E0771, n = 5; C57 BL/6 melanoma, n = 3; CaSki, n = 3). Data were collected from mice on a control diet for 4 weeks. (f) Percent of the indicated 18:1-containing PCs that incorporated 7 deuterium labels from the LPC 18:1-D7 tracer. Experiment was conducted by culturing CaSki cells (n = 3) with 20 μM LPC 18:1-D7 for 3 days. (g) Percent of the indicated 18:1-containing PCs in TC-1 tumours (n = 5) that incorporated 7 deuterium labels from the LPC 18:1-D7 tracer. See methods for detailed description of tracer administration. (h) 13C-labelling for a variety of LPC species within the tumour. Data are from C57 BL/6 mice (n = 4) harbouring E6/E7-expressing TC-1 subcutaneous tumours. Mice were given access to 5% [U-13C] fructose water for 120 h. Data were corrected for natural 13C abundance. All experiments from this figure were performed once. All data shown as mean ± standard deviation.
