Extended Data Fig. 1: Dietary fructose increases tumour growth without inducing metabolic syndrome in our models.
From: Dietary fructose enhances tumour growth indirectly via interorgan lipid transfer
(a) Schematic of zebrafish melanoma regrowth assay. Image created with Biorender.com. For (b-h), mice were administered a control diet (Ctrl), high-fructose diet (Frc diet), control diet + 10% fructose water (Frc), or control diet + 20% HFCS water (HFCS). Mice were administered the diets for 8 weeks (c,d) or 6 weeks (e-h). (b) Tumour volumes two weeks after HPV E6/E7-expressing TC-1 cells were orthotopically implanted into the cervix of C57 BL/6 mice (Ctrl, n = 4; Frc, n = 4; HFCS, n = 5). (c) Body weight of nude mice (n = 4 per condition). (d) Body weight of C57 BL/6 mice (Ctrl, n = 5; Frc, n = 5 and HFCS, n = 6). (e) Fasting serum glucose concentrations in nude mice (n = 5 per condition). (f) Fasting serum glucose concentrations in C57 BL/6 mice (n = 5 per condition). (g) Fasting serum insulin concentrations in nude mice (n = 4 per condition). (h) Fasting serum insulin concentrations in C57 BL/6 mice (n = 4 per condition). (i) Alanine tolerance test performed on C57 BL/6 mice after administering a control diet (Ctrl, n = 4) or a control diet + 10% fructose water (Frc, n = 4) for six weeks. Mice were gavaged with 0.5 g /kg [U-13C] alanine and serum 13C-glucose production was assessed. (j)-(m) Relative cell number (n = 5) after 4 days of culture at the indicated concentrations of insulin in DMEM + 0.1% FBS. All experiments in this figure were performed once. P values determined by ordinary one-way ANOVA with Tukey’s multiple comparisons test (b, e-h, j-m) or two-way ANOVA with Šídák’s multiple comparisons test (c, d, i). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and n.s. is not significant. Data in (b) shown as mean ± S.E.M. All remaining data shown as mean ± standard deviation.
