Extended Data Fig. 4: Addition of fructose to glucose-containing media does not enhance proliferation or alter metabolism.
From: Dietary fructose enhances tumour growth indirectly via interorgan lipid transfer
(a-l) Relative cell number after culturing the indicated cell lines in DMEM containing 20 mM glucose (Glc, n = 4-10) or 10 mM glucose + 10 mM fructose (Glc + Frc, n = 3-10) for 4 days. Media also contained 10% FBS and 1% pen/strep (m) Isotopologue distributions for the indicated central carbon metabolites from CaSki cells that were cultured with either 10 mM [U-13C] glucose (n = 3) or 10 mM [U-13C] glucose + 10 mM 12C-fructose (n = 3) for 4 h. 12C-fructose indicates unlabeled fructose. All experiments in this figure were performed once. P values determined by two-sided T-test (a-l) and multiple unpaired T-tests (m). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and n.s. is not significant. All data shown as mean ± standard deviation.
