Extended Data Fig. 5: Transformed cells produce endogenous fructose from glucose via fructoneogenesis.
From: Dietary fructose enhances tumour growth indirectly via interorgan lipid transfer
(a) Schematic depicting the polyol pathway and the generation of endogenous fructose from glucose. (b) Western blot analysis of sorbitol dehydrogenase from the indicated cell lines. (c-h) Isotopologue distribution for intracellular fructose after labelling the indicated cell lines with 5 mM [U-13C] glucose for 4 h (n = 3). (i) Fructose and glucose are chromatographically resolved with a pHILIC column (see Methods). Data in b are representative of two independent experiments. All other experiments were performed once. All data shown as mean ± standard deviation.
