Extended Data Fig. 5: Two-color in situ hybridization for validating Cre expression of transgenic lines.
From: Organ-specific sympathetic innervation defines visceral functions
Representative images of Cre and endogenous gene expression in the CG and SMG samples of RXFP1-Cre, SHOX2-Cre, TH-Cre and wild-type (WT) animals as indicated. Nuclei were visualized with DAPI staining (blue). Data were quantified from more than two mice per group. DP, double-positive. n = 5, 5, 8, 3 slices for RXFP1-Cre CG, RXFP1-Cre SMG, WT CG, and WT SMG. n = 5, 10, 2, 3 slices for SHOX2-Cre CG, SHOX2-Cre SMG, WT CG, and WT SMG. n = 5, 4, 2, 3 slices for TH-Cre CG, TH-Cre SMG, WT CG, and WT SMG. Scale bar, 100 μm. All data are shown as mean ± s.e.m.
