Extended Data Fig. 10: Measurements of GI transit.
From: Organ-specific sympathetic innervation defines visceral functions
a, Quantification of colored food transit after different feeding durations as indicated (n = 5 mice per group). b, Chemogenetic activation of CG-SMGTH neurons increased food consumption within the initial 10-min feeding after deprivation (n = 12 mice). c, Vehicle administration had no effects on food transit (n = 6 mice for WT, and 4 mice for SHOX2-Gq groups). d, At 30-min time point, activating CG-SMGSHOX2 neurons significantly slower food transit compared to wild-type control animals (n = 5 mice per group). e, Inhibition effects of chemogenetic stimulation of CG-SMGTH neurons on stool expulsion. The number of stools after either intraperitoneal PBS (Veh) or CNO injection is plotted (left) and quantified (right, n = 7 mice). f, Spontaneous stool defecation of TH-Cre control animals with AAV-FLEX-tdTomato injection to CG-SMG (n = 6 mice). g, Chemogenetic stimulation of CG-SMGTH neurons significantly suppressed spontaneous stool expulsion, which was reversed by the application of labetalol hydrochloride (n = 8 mice). h, Chemogenetic activation of CG-SMGTH neurons significantly delayed the total GI transit (n = 6 mice). *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed paired Student’s t-test or one-way ANOVA with Dunnett’s multiple comparisons test. Data are presented as mean ± s.e.m.
