Extended Data Fig. 7: Ageing changes DNA methylation patterns in primary AT2 cells and LUAD cells.

(a) Density plot showing Enzymatic Methyl-Sequencing (EM-Seq) coverage in primary AT2 cell samples. (b) Density plot of the methylation proportion in AT2 cell samples. (c) Heatmap showing unsupervised clustering of aged vs. young AT2 cells based on DNA methylation profiles. (d) Number of differentially methylated cytosines (DMCs) in aged vs. young AT2 cells based on location of CpG residues (n = 4 young and 4 age mice). (e) Density plot showing EM-Seq coverage in primary KP LUAD cell samples. (f) Density plot of the methylation proportion of KP LUAD samples. (g) Heatmap showing unsupervised clustering of aged vs. young KP LUAD cells based on DNA methylation profiles. (h) Number of differentially methylated cytosines (DMCs) in aged vs. young KP LUAD cells based on location of CpG residues (n = 5 young and 4 age mice). The central line within each boxplot in (d) and (h) represents the median (50^th percentile) and the boundaries of the box indicate 25^th and 75^th percentiles. The whiskers extend to the minimum and maximum values within 1.5 times the interquartile range (IQR) from the first and third quartiles, respectively. Points outside the whiskers are considered outliers and are shown as individual dots. Two-sided Wilcoxon rank-sum test was performed in (d) and (h).