Extended Data Fig. 12: Ageing-induced induction of NUPR1–lipocalin-2 axis is conserved in human lung and mouse intestine.
From: Ageing limits stemness and tumorigenesis by reprogramming iron homeostasis
(a) Quantification of AT2 cell density in healthy lungs of aged (>70 years old) and young or middle-aged (<50 years old) humans (n = 23 young/middle-aged and 25 aged patients, respectively). The proportion of HTII-280 positive AT2 cells (orange) per total lung cell number is shown. (b) Quantification of lipocalin-2 protein level in normal lung tissues of aged and young or middle-aged patients (n = 14 young/middle-aged cases vs. 15 aged cases). The intensity of lipocalin-2 (red) immunofluorescence was quantified only in cells expressing the AT2 cell marker SPC (green). A. U.: arbitrary units. (c) Quantification of Lcn2 mRNA (number of green dots) in aged vs. young mouse intestinal stem cells marked by the expression of Lgr5 (n = 291 Lgr5+ cells in the aged and n = 289 Lgr5+ cells in the young). (d) Quantification of organoid formation by intestinal crypts isolated from aged vs. young mice, with or without liproxstatin-1 and RSL-3 (n = 4 technical repeats per condition, a representative experiment repeated 3 times is shown). Scale bar: 20 µm in (a-c). Y: young; A: aged. Mean with SD is shown in (a-b) and (d). Mean with SEM is shown in (c). Two-tailed Student’s t test was used in (a-d).
