Extended Data Fig. 1: Single-cell analysis of prostate cancer patient biopsies identifies diverse cell types including multiple myeloid subsets.
From: Evolution of myeloid-mediated immunotherapy resistance in prostate cancer
(a-b) Bubble plots showing the relative expression levels of signature genes for (a) the indicated major cell types and (b) myeloid subsets. (c-d) (c) UMAP plot showing the enrichment scores for published FOLR2+ macrophage signatures and (d) heatmap depicting the normalized expression levels of FOLR2, SELENOP, and SLC40A1 in the indicated myeloid subsets. (e) Representative RNAscope images of HSPC and mCRPC patient biopsy samples. Immunostaining for SPP1hi-TAMs (SPP1; white, CD68; turquoise, and DAPI; blue) is presented. Scale bars, 30 μm. (f) Quantitative comparison of MDSC (P = 0.004) and SPP1hi-TAM (P = 0.34) frequency between scRNA-seq analysis versus tissue imaging. Bars show mean + SEM; symbols represent individual patients. (g) Bubble plot showing the relative expression levels of signature genes associated with immunosuppression. Statistical significance was determined by (f) two-sided paired Student’s t-tests; P-values: **P < 0.01. ns, not significant.
