Extended Data Fig. 7: Immunofluorescence assay of dRNH1 and 2xHBD in HSPCs.
From: Fetal hepatocytes protect the HSPC genome via fetuin-A
a, b, Representative fluorescence images and nuclear intensity of dRNH1 in HSPCs from E12.5 PL, E12.5 FL and E16.5 FL (n = 3). Scale bars, 10 and 5 µm. c, d, Representative fluorescence images and nuclear intensity of 2xHBD in HSPCs from E12.5 PL, E12.5 FL and E16.5 FL (n = 3). Scale bars, 10 and 5 µm. e, f, Representative fluorescence images and nuclear intensity of dRNH1 in HSCs and MPPs from E12.5 FL and E16.5 FL (n = 3). Scale bar, 10 µm. g, h, Representative fluorescence images and nuclear signal intensity of dRNH1 in E12.5 FL HSCs and MPPs cultured with or without FetuA (n = 3). Scale bar, 10 µm. The nuclear regions are circled by dotted lines (a, c, e, g). The medians (red dashed lines) and quartiles (black dashed lines) are shown (b, d, f, h). The n represents independent experiments. Statistical tests: unpaired two-sided Student’s t-test (b, d, f, h).
