Extended Data Fig. 8: Fetal liver HSPCs undergo DNA-replication and transcription simultaneously.
From: Fetal hepatocytes protect the HSPC genome via fetuin-A
a, b, Representative flow cytometry images and the proportions of each cell cycle stage in HSPCs from E12.5 PL, E12.5 FL and E16.5 FL cells stained with Hoechst 33342 and PyroninY (E12.5 PL, E16.5 FL, n = 3; E12.5 FL, n = 6; S/G2/M phase was compared). c, d, Representative flow cytometry images and the proportions of HSPCs in each cell cycle stage at E12.5 PL, E12.5 FL and E16.5 FL after 5-ethynyl-2’-deoxyuridine (EdU) treatment (n = 3 per group; S phase was compared). e, f, Representative flow cytometry images and Ki67-positive cell proportions in HSCs and MPPs from E12.5PL, E12.5FL and E16.5FL (n = 3 per group). g, h, Representative flow cytometry images and the ethyluridine (EU)-positive fraction of HSPCs from E12.5 PL, E12.5 FL and E16.5 FL after EU-treatment (E12.5 PL, E12.5 FL, n = 3; E16.5 FL, n = 6; EU+ population was compared). i, j, Representative fluorescence images and nuclear signal intensity of p-RPA in HSPCs from E12.5 PL, E12.5 FL and E16.5 FL (n = 3). The medians (red dashed lines) and quartiles (black dashed lines) are shown. Scale bar, 10 µm. k, l, Representative fluorescence images and nuclear signal intensity of p-RPA in E12.5 FL-HSPCs treated with or without FetuA (n = 3). The medians (red dashed lines) and quartiles (black dashed lines) are shown. Scale bar, 10 µm. m, n, Representative flow cytometry images and the proportion of each cell cycle stage in HSPCs cultured with or without FetuA and the BLM inhibitor ML216 (n = 3). The mean ± s.d. is shown (b, d, f, h, n). The n represents individual samples from 3 independent experiments (a-h) and independent experiments (i-n). Statistical tests: unpaired two-sided Student’s t-test (b, d, f, h, j, l, n).
