Extended Data Fig. 1: Flow-sorting of HSPCs and their sensitivity to genotoxic agents ex vivo.
From: Fetal hepatocytes protect the HSPC genome via fetuin-A
a, Immunophenotypically negative lineage (Lin−), positive Sca-l (Sca-1+) and Kit+ (LSK) cells were sorted by flow cytometry with high purities (mean ± s.d.) from the haematopoietic tissues of the E12.5 placenta (PL) (n = 4), E12.5 fetal liver (FL) (n = 6), E13.5 FL (n = 4), E14.5 FL (n = 4), E15.5 FL (n = 3), E16.5 FL (n = 3), E18.5 FL (n = 5) and perinatal bone marrow (BM) (n = 3) to assay the sensitivity of HSPCs to genotoxic agents. b, c, Representative fluorescence images and positive cell proportions of γ-H2AX etoposide (Eto)-induced ex vivo in HSPCs isolated from PL, FL and BM on different embryonic days (E13.5 FL, n = 4; E15.5 FL, n = 6; other groups, n = 3). Scale bar, 50 µm. d, e, Representative fluorescence images and positive cell proportions of γ-H2AX ultraviolet (UV) light-induced HSPCs isolated from PL, FL and BM on different embryonic days (E12.5 PL, Perinatal BM, n = 4; E12.5 FL, E16.5 FL, n = 5; E13.5 FL, n = 9; E14.5 FL, E18.5FL, n = 3; E15.5 FL, n = 6). Scale bar, 50 µm. The n represents independent experiments. The mean ± s.d. is shown (c, e). Statistical tests: unpaired two-sided Student’s t-test (c, e).
