Extended Data Fig. 3: Chromosomal alterations in BM HSPCs from Eto-treated mice at the embryonic stage.
From: Fetal hepatocytes protect the HSPC genome via fetuin-A
a, Experimental design for whole-genome sequencing (WGS) and chromosome fluorescence in situ hybridization (FISH). b, The genome mutation numbers in 3-week-old BM-LSK cells (E12.5 FL, n = 3; E16.5 FL, n = 4). The boxes delimit the minima and maxima, and the horizontal line represents the mean. c, d, Representative fluorescence images and proportions of normal and aberrant chromosomes in cells from mice treated with Eto at E12.5 or E16.5, chromosome 4 (FITC) and chromosome 6 (Texas red); arrows indicate aberrant chromosomes (Untreated, n = 6, E12.5 Eto, n = 15; E16.5 Eto, n = 5; mean ± s.d.). Scale bar, 10 µm. The n represents individual samples (b) and mice (c, d) from 3 independent experiments. Statistical tests: unpaired two-sided Student’s t-test (b), unpaired one-sided Student’s t-test with Welsh’s correction (d).
