Extended Data Fig. 7: Structural bases of kinase anchoring to radial spokes.
From: Structural diversity of axonemes across mammalian motile cilia
(a) Overview showing the positions of kinases (colored) in the radial spokes of the bovine sperm DMT. (b) Interactions of adenylate kinase 8 (AK8) with PPIL6 and DNAJB13 stabilize the open conformation of catalytic domain II (left). AK8 is anchored in the neck of RS1 and RS2 via its N-terminal dimerization/docking domain, which interacts with EFCAB10 and packs against RSPH3 (right). (c) AK7 and AK9 are anchored to RS3 via their C-terminal DPY30 domains. The DPY30 domain of AK7-A dimerizes with the DPY30 motif in EFCAB5, while the DPY30 domain of AK7-B interacts with AK9; both pairs of proteins then dock onto CFAP91. (d) CAMK4 interacts with RSPH1, DYDC2, and PPIL6 in the heads of RS1 and RS2. See also the Supplementary Fig. 14. (e) The ciliary PKA holoenzyme is a heterodimer of a catalytic (PKAC) and regulatory (PKAR) subunit. PKAR interacts with a malate dehydrogenase dimer (MDH1A/MDH1B) in the center of the RS3 head.
