Extended Data Fig. 8: latSC neuronal activity can be selectively tuned to contact events on some licks, but not others.
a-b, Example latSC neurons tuned to contacts on specific licks. a, A neuron tuned to certain contact locations during L2, but not L4. b, A neuron tuned to certain contact locations during L4, but not L2. c, Left, Percentage of L2- and L4-modulated neurons tuned to contacts on L2 only (dark green), L4 only (light green), both L2 and L4 (green), or neither L2 or L4 (grey). Right, Pearson’s R between L2 contact angle and mean firing rate following L2 contact plotted against Pearson’s R for L4 contact angle and mean firing rates following L4 contact. Positive and negative correlation coefficients indicate right or left preference, respectively. Note that the activity of many latSC neurons exhibited similar correlations between firing rate and contact location on L2 and L4. d, Example latSC neuron selective for L2 centre contacts that was re-tuned to non-centre responses on L4 during recentring trials. e, Example L4 modulated neuron that was activated by both left and right recentring trials. f, Left, Percentage of neurons selective for L2 centre contacts that were re-tuned to a non-centre contact on L4 (blue). Blue and grey, re-tuned and not re-tuned, respectively. Right, Percentage of L4 modulated neurons that were activated by both left and right recentring trials (orange). g, Left, Illustration of a hypothetical conjunctively-tuned neuron. Right, Spike rasters and rate histograms for an example conjunctively-tuned latSC neuron aligned to L2 (left) and L4 contact (right). Colours as in Fig. 1a. h, Median ± IQR of single neuron divergence latencies between left, centre and right conditions for tongue-, head-, and conjunctively-tuned neurons, as well as neurons that were not correlated with any reference frame. Note that tactile responses in neurons with a head-centric reference frame diverged significantly later than those with tongue-centric (p = 0.003) or conjunctive (p = 0.007) reference frames, as well as neurons that were modulated, but not correlated (p = 0.006), with contact site. Grey dots are individual neuron divergence latencies. *corrected p < 0.05, **corrected p < 0.01, one-sided hierarchical bootstrap test. For all rate histograms, shading represents the bootstrapped s.e.m. across trials. g was adapted from ref. 8 (Springer Nature Limited).
