Extended Data Fig. 7: Ciliated endothelium noted within metaphyseal capillaries.
From: Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance
a, Tissue scanning electron microscope images of whole-mounted long bones. By zooming in whole-mounted long bones (upper right), metaphyseal BM regions within ~150 µm from the endosteum surface at the epiphyseal line were identified. The lowest bottom two pictures demonstrate fine capillaries (<2–4 µm in diameter) branching from bigger blood vessels (over 10 µm in diameter). A total of six bones were imaged. Total six independent experiments. The size of the scale bar was indicated in each picture. b, Representative tissue scanning electron microscope images of ciliated structures on metaphyseal capillaries. Ciliated structures project into the lumen of metaphyseal capillaries. The lengths are 1 to 10 µm. The size of the scale bar was indicated in each picture. Refer to Supplementary Video 4. A total of six bones were imaged. Total six independent experiments. c, Representative electron microscope image of diaphyseal sinusoids (over 100 µm in diameter). The ciliated structures were not found on the sinusoid endothelium. The size of the scale bar was indicated in each picture. A total of six bones were imaged in six independent experiments.
