Extended Data Fig. 1: Flow cytometric analysis of NO^Hi HSCs.

a, NO staining. MFI of DAF-FM in different BM fractions. LSK: Lin⁻Sca1⁺cKit⁺. Lin⁻: Lin⁻ BM cells. Lin⁺: Lin⁺ BM cells. CD11b⁺: CD11b⁺ BM cells. CD11c⁺: CD11c⁺ BM cells. Treg: FoxP3⁺CD4⁺CD3⁺NK1.1⁻ BM Tregs. non-Treg: FoxP3⁻CD4⁺CD3⁺NK1.1⁻ BM T cells. NK cell: NK1.1⁺CD3⁻ BM NK cells. CD45⁻: CD45⁻Ter119⁻ mesenchymal cells. N = 3. The results were analysed by 1-way ANOVA with post hoc test with Bonferroni correction. b, Expression levels of CD200R in NO^Hi, NO^LowCD200R⁺, or NO^LowCD200R⁻ HSCs. Left: representative flow cytometry plots illustrating the gating strategy to identify NO^Hi, NO^LowCD200R⁺, or NO^LowCD200R⁻ HSCs within CD150⁺CD48⁻Lin⁻Sca1⁺cKit⁺ HSCs. Right: representative histograms. Red: NO^Hi HSCs. Blue: NO^LowCD200R⁺ HSCs. Orange: NO^LowCD200R⁻ HSCs. c, Ratios of CD200R MFIs to the average of MFIs in NO^LowCD200R⁻ HSCs. The results were pooled from two independent experiments and analysed by 1-way ANOVA with post hoc test with Bonferroni correction (total N = 8/each). d, NOS and CD150⁺CD48⁻Lin⁻Sca1⁺cKit⁺ HSCs. Flow cytometry data show correlations of NO levels with eNOS and nNOS.

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