Extended Data Fig. 2: NO expression indicates robust but late-rising HSCs.
From: Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance
a, Serial transplantation assays. NOHi, NOLowCD200R+, or NOLowCD200R− HSCs (10 cells/recipient; B6 SJL; CD45.1) were injected into tail veins of lethally irradiated B6 congenic recipient mice (CD45.2), together with competitor B6 BM cells (200,000 cells/recipient; CD45.2). Serial transplantation was performed twice at five-month intervals by transplanting BM cells (5 × 106/each) of the recipient mice into irradiated B6 recipient mice (950cGly). Refer to Fig. 1g–k. b, CD45.1+ donor blood chimerism in B6 recipients transplanted with NOHi, NOLowCD200R+, or NOLowCD200R− HSCs (B6 SJL). Donor chimerism was analysed monthly. The results of the first and secondary transplantation are pooled from three independent experiments (three donors in total; 17–20 recipients per group). The third transplantation experiments includes 6–8 recipients/group. Data were analysed by 1-way ANOVA with post hoc test with Bonferroni correction. 1st: first transplantation. 2nd: secondary transplantation. 3rd: third transplantation. c, Differential patterns of blood reconstitution derived from NOHi, NOLowCD200R+, or NOLowCD200R− HSCs. Reconstitution patterns were categorized as in Extended Data Fig. 2d. d, Differential reconstitution patterns. e, Trend of CD45.1+ donor chimerism over the 1st, 2nd and 3rd transplantation. Experiments include 6–8 recipients/group. The colours of the trend lines correspond to those of the differential reconstitution patterns shown in Extended Data Fig. 2d. f, Lineage skewing analyses. These were done using CD45.1+ donor blood cells in B6 recipient mice at 16 weeks after the transplantation of NOHi or NOLowCD200R+ HSCs (B6 SJL). N = 5–10. The results were pooled from three independent experiments. Frequencies of myeloid cells, T cells, and B cells in CD45.1+ donor blood cells were analysed by two-sided Student’s t-test. All data are presented as mean ± SD. Asterisks indicate significant differences (p-value < 0.05, *; <0.01, **; <0.001, ***; <0.0001, ****; N.S., >0.05).
