Extended Data Fig. 1: Soluble SNIPR tunability and specificity.
From: Engineered receptors for soluble cellular communication and disease sensing
a, BFP reporter induction in a panel of SNIPR variants bearing scFvs targeted against TGFβ (left) or VEGF (right) in VHVL or VLVH orientation in primary human T cells. n = 3 (left) and 2 (right) technical replicates. b, BFP reporter gene induction of primary human T cells expressing SNIPRs containing a set of unique Transcription Activation Domains (TADs) (n = 2 technical replicates). c, BFP reporter induction by conventional or hinge-mutant (hm) TGFβ and VEGF SNIPR or untransduced T cells (n = 3 technical replicates). Statistics represent difference between SNIPR and hmSNIPR variants computed via two-way ANOVA with Šídák’s multiple comparisons test. d, BFP reporter activation by TGFβ (left) and VEGF (right) SNIPRs in primary T cells from three different donors (n = 3 technical replicates per donor). e, Activation of TGFβ SNIPR and VEGF SNIPR T cells by their matched or mismatched ligands, respectively (n = 3 technical replicates). Statistics calculated using unpaired two-tailed Welch’s t-test. f, TGFβ SNIPR activation in primary human T cells using recombinant active TGFβ, recombinant latent TGFβ, or recombinant latent TGFβ re-activated via heat exposure (n = 3 technical replicates). g, TGFβ SNIPR activation in primary human T cells using recombinantly expressed human TGFβ1, β2, or β3 protein isoforms (n = 3 technical replicates).
