Extended Data Fig. 7: H3Q5his and H3K4me3Q5his display diurnal rhythmicity in TMN, but not SCN.
From: Bidirectional histone monoaminylation dynamics regulate neural rhythmicity
a,c, H3Q5his and H3K4me3Q5his in (a) TMN, but not (c) SCN (H3Q5his only), display a rhythmic pattern of expression across ZT in mice [TMN – H3K4me3Q5his: ZT0/ZT4/ZT8, n = 9 biological replicates per time point; ZT12/ZT16/ZT20, n = 10 biological replicates per time point; TMN – H3Q5his: ZT0/ZT4/ZT12/ZT20, n = 9 biological replicates per time point; ZT8/ZT16, n = 7 biological replicates per time point; SCN – H3Q5his: ZT0/ZT4/ZT8/ZT12/ZT16/ZT20, n = 9 biological replicates per time point). Comparison of fits analysis between third order polynomial, cubic/rhythmic trend (alternative hypothesis) vs. first order polynomial, straight line/linear trend (null hypothesis): TMN H3K4me3Q5his – p = 0.0428, null hypothesis rejected; TMN H3Q5his – p = 0.0135, null hypothesis rejected; SCN H3Q5his – p = 0.8500, null hypothesis not rejected. Data additionally analysed via one-way ANOVA (TMN H3K4me3Q5his: p = 0.0181, F5,51 = 3.029; TMN H3Q5his: p = 0.0066, F5,40 = 3.796; SCN H3Q5his: p = 0.8077, F5,42 = 0.454) with Tukey’s MC tests. Statistical differences between time points are noted [TMN H3K4me3Q5his: *p = 0.0317 (ZT4 vs. ZT8), *p = 0.0133 (ZT4 vs. ZT16); TMN H3Q5his: *p = 0.0108 (ZT4 vs. ZT12), *p = 0.0381 (ZT12 vs. ZT20)]. Data presented as mean +/– SEM. b, TG2 in TMN does not display rhythmic fluctuations in expression across ZT (ZT0/8/12/16, n = 8 biological replicates per time point; ZT4/20, n = 7 biological replicates per time point). Comparison of fits analysis between third order polynomial, cubic/rhythmic trend (alternative hypothesis) vs. first order polynomial, straight line/linear trend (null hypothesis): – p = 0.5718, null hypothesis not rejected. Data additionally analysed via one-way ANOVA (p = 0.2246). Supplementary Fig. 1 = uncropped blots. Source data provided as a Source Data file.
