Extended Data Fig. 8: Dynamic epigenomic regulation of H3 monoaminylations vs. H3K4 methylation across ZT.
From: Bidirectional histone monoaminylation dynamics regulate neural rhythmicity
a, Neither Hdc nor NeuN levels display rhythmic regulation across ZT in TMN (n = 3 biological replicates/time point). Data analysed via one-way ANOVA (Hdc: p = 0.9132, F5,12 = 0.2835; NeuN: p = 0.3223, F5,12 = 1.313). SCN was used as a negative control to monitor the accuracy of dissections for TMN tissues. b,c, Neither (b) histamine nor (c) serotonin levels (total concentrations, not exclusively intracellular) in TMN display rhythmic regulation across the ZT in TMN (n = 3 biological replicates/time point). Data analysed via one-way ANOVA (histamine ELISA: p = 0.9901, F5,12 = 0.1007; serotonin ELISA: p = 0.7543, F5,12 = 0.5236). d–i, Heatmaps displaying enrichment for (d) H3K4me2, (e) H3K4me3, (f) WDR5 and (g) H3K4me3Q5ser across ZT at genic loci in TMN. Data were normalized to both (h) IgG (the signal of which was not appreciable in TMN) and E. coli spike-in DNA; (i) note that without appropriate normalization to E. coli spike-in DNA, rhythmic patterns of H3K4me3Q5his are largely not observed. See Supplementary Tables 3 and 4 for rhythmic analyses of H3K4me3Q5his and H3K4me3Q5ser. Bar graphs presented as mean +/– SEM. Supplementary Fig. 1 = uncropped blots. Source data provided as a Source Data file.
