Extended Data Fig. 1: Conformational transition differences in MILI ternary complexes compared to hAGO2 and TtAGO ternary complexes.
From: Structural insights into RNA cleavage by PIWI Argonaute
a,b, Stereo views of ternary complexes of mouse PIWI Argonaute MILI and 26-nt guide RNA with target RNAs of 15 nt (PDB: 7yfy) (a, left) and 26 nt (full complementarity) (b, left), human AGO2 (hAGO2) and 21-nt guide RNA with target RNAs of 16 nt (PDB: 6n4o) (a, middle) and 21 nt (mismatches at position 10-11) (PDB: 6mdz) (b, middle), T. thermophilus AGO (TtAGO) and 21-nt guide DNA with target RNAs of 15 nt (PDB: 4n41) (a, right) and 19 nt (PDB: 4ncb) (b, right). c, Superposition of MILI ternary complexes with 15-nt-target and 26-nt-target conformations (c, left), hAGO2 ternary complexes with 16-nt-target and 21-nt-target conformations (c, middle), TtAGO ternary complexes with 15-nt-target and 19-nt-target conformations (c, right). d,e, Cross-sectional views of the MILI ternary complexes with 15-nt-target (d, left) and 26-nt-target conformations (e, left), hAGO2 ternary complexes with 16-nt-target (d, middle) and 21-nt-target conformations (e, middle), TtAGO ternary complexes with 15-nt-target (d, right) and 19-nt-target conformations (e, right). f, Superposition of cross-sectional views showing the rotation of PAZ-domain in MILI ternary complexes with 15-nt-target and 26-nt-target conformations (f, left), hAGO2 ternary complexes with 16-nt-target and 21-nt-target conformations (f, middle), TtAGO ternary complexes with 15-nt-target and 19-nt-target conformations (f, right). Arrows indicate major shifts of PAZ and N domain in two different conformations.
