Extended Data Fig. 2: Structural comparison between MILI-guide-target complexes and hAGO2/TtAGO-guide-target complexes.
From: Structural insights into RNA cleavage by PIWI Argonaute
a, d, g, Conformational superposition of MILI-guide RNA complex binding target RNAs with complementarity to nucleotides g2-g15 and g2-g26 (a), hAGO2-guide RNA complex binding target RNAs with complementarity to nucleotides g2-g16 and g2-g21 (d), TtAGO-guide DNA complex binding target RNAs with complementarity to nucleotides g2-g15 and g2-g19 (g). b, e, h, Schematic of major contacts between the guide (red) and target (blue) and MILI in the g2-g15 base-paired conformation (b), and hAGO2 in the g2-g16 base-paired conformation (e), and TtAGO in the g2-g15 base-paired conformation (h). c, f, i, Schematic of major contacts between the guide (red) and target (blue) and MILI in the g2-g26 base-paired conformation (c), and hAGO2 in the g2-g21 base-paired (g10-g11 mispairing, colored by light blue) conformation (f), and TtAGO in the g2-g19 base-paired conformation (i). Residues colored by domain, as in Fig. 2. Disordered nucleotides labelled by dashed circle.
