Extended Data Fig. 3: Biochemical determination of target cleavage by MILI mutants.
From: Structural insights into RNA cleavage by PIWI Argonaute
a-f, Fraction of 2-26 target RNA (10 nM) cleaved by wild-type (100 nM) or MILI mutants versus time. L1-domain (S379A, H380A, K381A) mutant (a), PAZ-domain (T424A, T499A) mutant (b), L1- and PAZ-domain (S379A, H380A, K381A,T424A, T499A) mutant (c), L1-hairpin (R339G, N340G) mutant (d), PIWI-cS7 (S816A, D817A, Q819A) mutant (e), both N-domain (K252A, D273A, S275A (termed as N1)) and (Q240A, H242A, T307A, K308A (termed as N2)) mutants (f). g, t10-t11 docking in the active site of MILIWT-piRNA-target (26 nt) complex (left) and MILIR853A-piRNA-target (26 nt) complex (right). Plotted data are represented as mean ± s.d. from independent triplicates.
