Extended Data Fig. 4: Cryo-EM Structural comparison of EfPiwi-piRNA-target (25 nt) and MILI-piRNA-target (26 nt) complexes in the “comma-shaped” and “locked” conformations.
From: Structural insights into RNA cleavage by PIWI Argonaute
a,b, Cryo-EM structures of EfPiwi-piRNA bound to target RNA (25 nt) (a) and MILI-piRNA complex bound to target RNA (26 nt) (b). c,d, Superposition of guide-target RNA duplex and adjacent PIWI-MID domain in the EfPiwi ternary complex with 25 nt (purple) in the “comma-shaped” conformation and in the MILI ternary complex with 26-nt target RNA (blue) in the “locking” conformation (c). Insert shows the conformational difference within catalytic pocket (d). Black arrow indicates the shift of the RNA duplex and the scissile phosphate from the “comma-shaped” to the “locked” ternary complexes, respectively.
