Extended Data Fig. 9: Specificity and K_M,RuBP measurements for A102Y and V266T.

a) Specificity values measured by Membrane Inlet Mass Spectrometry (N = 3 for each mutant measured in this study). Comparisons to literature values are displayed when available. Literature data for WT is from³⁹. Error bars represent the SEM of all measurements compiled in that published analysis. Literature data for H44N and D117V is from²⁴. Error is taken from Extended Data Table 2 in that publication. P-values reflect a Welch’s two-sided t-test in comparison to WT, with a permutation test to determine P-values. Red numbers indicate P > 0.05. b) K_M,RuBP values fit from spectrophotometric assays of rubisco carboxylation along an 8 point RuBP titration. Each point in the titration was measured in technical triplicate. Error bars indicate the square root of the diagonals of the covariance matrix during fitting. All three triplicate measurements were used to perform the fit.