Fig. 4: Single-amino acid mutations can traverse the functional landscape.
a, \({\widetilde{K}}_{{\rm{C}}}\) versus effect size for each mutant. Effect size is the difference between the mutant \({\widetilde{K}}_{{\rm{C}}}\) and WT KC divided by the coefficient of variation of \({\widetilde{K}}_{{\rm{C}}}\). b, PDB structure 9RUB; inset on the C2 symmetry axis is expanded below. Each position appears twice due to proximity to the C2 axis. c, kcat versus KC of the indicated mutants (as measured by 14C assay) versus all measured rubiscos from refs. 6,12). Shaded regions indicate known ranges of \({\widetilde{K}}_{{\rm{C}}}\) values for plants and algae in green and Form II bacterial rubiscos in pink. Star, WT R. rubrum; triangles, mutants A102Y and V266T.
