Extended Data Fig. 8: Aap-sc-TTFC elicit potent antibody response against TTFC systemically and at mucosal surface.
From: Discovery and engineering of the antibody response to a prominent skin commensal
a, Experimental design. To ensure that conjugation of TTFC to the bacteria (i.e. Aap-sc) was necessary to induce antibodies against TTFC, we tested whether application of TTFC topically alone was sufficient to induce a response against TTFC. To identify the right amount of TTFC to apply, we first quantified the total amount of antigen given to mice when provided as Aap-sc-TTFC (see Extended Data Fig. 7). We estimated that each Aap-sc bacterium was conjugated to 30–60,000 TTFC molecules, corresponding to ~1 μg of TTFC per mouse per colonization. For topical administration, we thus used 5 μg of TTFC per application to be conservative. b, From top to bottom: TTFC-specific IgG titres in the serum, IgG in bronchoalveolar lavage (BAL) fluid, IgA in BAL fluid, and IgA in nasal washes for 13 (left), 3 (middle) or 2 (right) colonizations. c, Colony-forming units (CFUs) for the mice shown above in b and in Fig. 4f. All mice shown from two independent experiments.
