Extended Data Fig. 3: Identification of the minimal epitope in the B domain of Aap.
From: Discovery and engineering of the antibody response to a prominent skin commensal
a, Schematic of LM088 Aap protein truncations for expression in E. coli. b, Immunoblot analysis of the constructs shown in a using anti-HA antibody to assess expression (induced by IPTG), using serum from naive mice or mice colonized with LM088. (1 = amino acid repeat domain, 2 = parallel β-helix domain, and 3 = one repeat of the B domain). c, Schematic of the B domain truncations fused to superfolder GFP (sGFP) for expression in E. coli. d, Immunoblot analysis of the truncations shown in c using anti-HA antibody or the serum of mice colonized with LM088. e, Minimal epitope identified in the B domain and the peptides synthesized and tested in f. f, Dot blot analysis of the biotinylated peptides shown in e using streptavidin-HRP or the serum of mice colonized with LM088. All blots are representative of three independent experiments. g, Immunoblot of cell lysate (L) and culture supernatant (S) of LM088 and LM088 Δaap using serum from mice colonized with LM088 or LM088 Δaap (from the mice shown in Fig. 2j). Representative of two independent experiments. Uncropped immunoblot and dot blot images can be found in Supplementary Figs. 2b–d and 3a,b.
