Extended Data Fig. 4: Redirecting the B cell response to S. epidermidis using engineered strains.
From: Discovery and engineering of the antibody response to a prominent skin commensal
a, 6–10-week-old SPF mice were colonized for 6 weeks with S. epidermidis strain recombinantly expressing different tetanus toxin fragment C (TTFC) fusion proteins. The constructs were built in either WT LM087 (black) or LM088 Δaap (purple) background strain. b, Serum titres against TTFC six weeks post colonization, and c, TTFC specific IgA in nasal washes (undiluted washes). N = 8/group, two independent experiments pooled. Of note, for clarity, panel b shows medians and panel c displays means. The dashed lines show the corresponding median (b) and mean (c) values for strain Δaap + Aap-TTFC (wTTFC) (see Fig. 3d, which was run together with the data shown in Extended Data Fig. 4). d,e, Colony-forming units (CFUs) at the experimental endpoint for the constructs described in a on BHI (d) or BHI chlor (e). p = peptidoglycan targeting (i.e. LysM-2x TTFC). LOD = limit of detection.
