Extended Data Fig. 8: Toxic genes of phage origin do not activate the Lysobacter gasdermin system.
From: CARD domains mediate anti-phage defence in bacterial gasdermin systems
a. Bacteria co-expressing the Lysobacter gasdermin system with toxic genes of phage origin that are unrelated to RIIB (Supplementary Table 3) were plated in 10-fold serial dilution on LB-agar plates in conditions that repress expression (1% glucose) or induce expression (0.2% arabinose and 1 mM IPTG). Negative control represents cells expressing GFP instead of the gasdermin system. b. Expression of toxic phage genes does not lead to gasdermin cleavage. Western blot analyses of N-terminally HA-tagged gasdermin co-expressed with toxic genes of phage origin, before (0 min) and 80 min after the expression of the toxic gene was induced. GroEL was used as a loading control.
