Extended Data Fig. 5: Lysobacter NLR-like protein encodes a CARD domain at the C-terminus.
From: CARD domains mediate anti-phage defence in bacterial gasdermin systems

a. An AlphaFold2 model of the Lysobacter NLR-like ATPase protein. b. An AlphaFold2 model of the C-terminal domain of the NLR-like ATPase. The human ICEBERG CARD domain (PDB ID 1DGN) is shown for comparison. c. A plot of DALI Z-scores of protein structures similar to the Lysobacter NLR-like CARD domain structure predicted by AlphaFold2. d. Deletion of the C-terminus of the NLR-like gene in the Lysobacter gasdermin system leads to toxicity. Bacteria expressing the WT or CARD-deleted Lysobacter gasdermin system were plated in 10-fold serial dilution on LB-agar plates in conditions that repress expression (1% glucose) or induce expression (0.2% arabinose).