Extended Data Fig. 7: Mapping the leptin-melanocortin pathway: MC3R and MC4R.

a-b, Log-normalized spatial expression of MC3R (a) and MC4R (b) in the human hypothalamus ST sections. c-d, UMAP expression plots (log-normalized) showing MC3R (c) and MC4R (d) in the snRNA-seq dataset. e, Clusters expressing MC4R or MC3R in either human or mouse. Left: The dendrogram depicts the top 5% of all human C4 clusters expressing MC4R or MC3R and their ancestors on level C1-3. Edges are labelled with cluster names. Regional annotations (if applicable) are annotated next to each C4 cluster. Right: The highest correlated mouse cluster (if available) is shown. The small number indicates the total number of matched mouse clusters to the human C4 cluster and is coloured by correlation of the highest cluster. Middle: The outer heatmaps depict the average expression of selected marker genes in human (orange) or mouse (green) clusters. The inner heatmaps depict the average expression of MC4R and MC3R in human (orange) or mouse (green) clusters. f, Cell abundance scores from cell2location of 5 MC3R-expressing clusters (C4-161, C4-390, C4-391, 4-161, C4-64) in a medial section of the human hypothalamus.