Extended Data Fig. 3: Characterization of peripheral immune responses to vaccination.
From: A neoantigen vaccine generates antitumour immunity in renal cell carcinoma
a, Example IFNγ ELISpots for selected driver mutations, including SNVs (PIK3CA and BAP1) and indels (KDM5C and PBRM1). b, Gating strategy for flow cytometry assessment of T cell cytokine production and phenotype. Example flow cytometry gating strategy for identifying CD4+ and CD8+ T cells, and production of IFNγ, TNFα, and IL-2 cytokines in those T cell subsets. c, Expression of CD45RO and, d, PD-1 on CD4+ IFNγ+ (vaccine-reactive) T cells (as measured by the median fluorescence intensity; MFI) after stimulation with vaccine peptide pools.
