Extended Data Fig. 2: Focused validation of PELO dependency.
From: SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent on PELO
(a) Relative viability normalizing to negative control, with mean of positive controls per cell line represented by horizontal red lines. (b) Immunoblot of PELO and Vinculin levels in indicated cell lines 4-6 days following transduction of indicated gRNAs. (c) Immunoblot of PELO and β-actin levels in engrafted MIA PaCa-2 tumors n = 1 mice on standard diet (DOX−) or n = 3 mice on DOX-containing diet (DOX+). DOX+ tumors were harvested at indicated times following the start of the diet. (d) Tumor volume plotted by time following randomization for individual MIA PaCa-2 xenograft growth ± DOX-induced PELO KD, showing all tumors (n = 19). All DOX-treated mice resumed a standard diet 66 days following randomization. Data are mean ± s.e.m. of biological replicates: n = 6 for all cell lines except KP4 and GB1 where n = 7 and IGROV-1 where n = 3 in (a), and n = 9 tumors on standard diet and n = 10 for DOX-containing diet in (d). n = 4 mice for (c). Significance was calculated as follows: (a) left-tailed Student’s t-test. Representative data from two experiments in (a,b) and one experiment in (c,d). Experiments were performed twice for (a,b) and once for (c,d). For immunoblots, Vinculin and β-actin were used as loading controls.
