Extended Data Fig. 5: Lepr-CreERT2 labeled alveolar fibroblasts generate pathological fibroblasts.
From: RUNX2 promotes fibrosis via an alveolar-to-pathological fibroblast transition
a, The cell distribution of Lepr-CreERT2 labeled fibroblast clusters along with the color-coded pseudotime (upper panel). Heatmap showing dynamic gene expression in fibroblast clusters (lower panel). Representative genes (left) and enriched pathways (right) in alveolar fibroblasts and pathological fibroblasts are shown, respectively. b, The expression levels of the alveolar fibroblast makers Scube2, Npnt, Inmt and the pathological fibroblast markers Cthrc1, Postn along pseudotime analysis. c-d, The top ten GO terms (c) and KEGG pathways (d) associated with genes enriched in pathological fibroblasts. e, UMAP plot showing the enrichment of Tgfβ1 transcript in pathological fibroblasts. f, UMAP plot showing the expression of Lepr transcript in lung mesenchymal cells of Lepr-CreERT2;R26tdT mice challenged with saline or bleomycin.
