Extended Data Fig. 9: Conditional deletion of Runx2 attenuates bleomycin-induced lung fibrosis.
From: RUNX2 promotes fibrosis via an alveolar-to-pathological fibroblast transition
a,b, FACS analysis to quantify bone marrow monocytes among CD45+ cells (Lepr-Cre;Runx2+/+: n = 3, Lepr-Cre;Runx2f/f: n = 3). c,d, FACS analysis to quantify bone marrow neutrophils among CD45+ cells (Lepr-Cre;Runx2+/+: n = 3, Lepr-Cre;Runx2f/f: n = 3). e, Representative H&E staining images of untreated lungs from Lepr-Cre;Runx2+/+ and Lepr-Cre;Runx2f/f mice. f, Experimental schematic and representative H&E staining images of the lungs from Lepr-Cre;Runx2+/+ and Lepr-Cre;Runx2f/f mice after bleomycin challenge. g, Quantification of fibrotic areas in the lungs of Lepr-Cre;Runx2+/+ (n = 9) and Lepr-Cre;Runx2f/f mice (n = 9). h, Hydroxyproline content in the lungs of Lepr-Cre;Runx2+/+ (n = 7) and Lepr-Cre;Runx2f/f mice (n = 7) treated with bleomycin. i, α-SMA and SPC expression in the lungs of Lepr-Cre;Runx2+/+ and Lepr-Cre;Runx2f/f mice after bleomycin challenge. j, Quantification of α-SMA+ cells in the lungs of Lepr-Cre;Runx2+/+ (n = 6) and Lepr-Cre;Runx2f/f mice (n = 6). k, Experimental schematic and representative Picro-Sirius Red staining images. l, α-SMA and tdT expression in the lungs of Lepr-CreERT2;Runx2+/+;R26tdT and Lepr-CreERT2;Runx2f/f;R26tdT mice. m, Quantification of α-SMA+ cells in the lungs of Lepr-CreERT2;Runx2+/+;R26tdT (n = 6) and Lepr-CreERT2;Runx2f/f;R26tdT mice (n = 6). n, Experimental schematic and representative Picro-Sirius Red staining images. o, α-SMA and tdT expression in the lungs of Scube2-CreERT2;Runx2+/+;R26tdT and Scube2-CreERT2;Runx2f/f;R26tdT mice. p, Quantification of α-SMA+ cells in the lungs of Scube2-CreERT2;Runx2+/+;R26tdT (n = 6) and Scube2-CreERT2;Runx2f/f;R26tdT mice (n = 6). Data are representative of at least three independent experiments. Data are mean ± SEM. Statistical analysis was performed using unpaired two-tailed t-test with Welch’s correction. Scale bars: e, f, k and n:1 mm (e and f: 200 µm in magnified views, k and n: 100 µm in magnified views), i, l and o: 100 µm (20 µm in magnified views).
