Extended Data Fig. 8: Neoantigen-reactive T-cell clones are isolated from PBMC and elicit an immune response upon neoantigen recognition. | Nature

Extended Data Fig. 8: Neoantigen-reactive T-cell clones are isolated from PBMC and elicit an immune response upon neoantigen recognition.

From: Tumour-wide RNA splicing aberrations generate actionable public neoantigens

Extended Data Fig. 8

A. Pipeline for validating the specificity of neoantigen-reactive TCR clonotypes found in 10x V(D)J single-cell RNA-seq (scRNA-seq) against NJ-derived neoantigen candidates utilizing a TCR-transduced triple-reporter Jurkat76/CD8 system followed by flow cytometry analysis. B-D. NEJGNAS-derived and NEJRPL22-derived neoantigen-specific TCR-transduced triple-reporter Jurkat76 cells activated against dose-dependent neoantigen-pulsed T2 cells. TCR activation of triple-reporter TCR-transduced triple-reporter Jurkat76 is measured by flow cytometry analysis of (B) NFAT-GFP, (C) AP-1-mCherry, and (D) NFκB-CFP. a, Created in BioRender (credit: D.W.K., https://BioRender.com/z56q380; 2024).

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