Extended Data Fig. 9: High-affinity neoantigen-reactive T-cell receptors recognize cancer-specific neoantigen sequences.
From: Tumour-wide RNA splicing aberrations generate actionable public neoantigens
A. TNFα expression of PBMC-derived CD8+ T-cells (n = 3) transduced with TCRG4.1 (top) and TCRR3.9 (bottom) against T2 cells pulsed with varying concentrations of corresponding neoantigen or decoy antigen. B. Alanine scanning mutagenesis of NeoAGNAS (top) and NeoARPL22-reactive (bottom) TCR-transduced triple-reporter Jurkat76/CD8 cells co-cultured with alanine-substituted neoantigen-pulsed T2 cells (n = 3), neoantigen-pulsed T2 cells (n = 3), or non-pulsed T2 cells (n = 3). Flow analysis was performed to evaluate TCR activity through NFAT-GFP (left), AP-1-mCherry (center), and NFκB-CFP (right) activity.
