Extended Data Fig. 10: Early stem-like CD8+ T cells from acute viral infection become chronic resource CD8+ T cells after transfer into chronically infected mice.
From: An early precursor CD8+ T cell that adapts to acute or chronic viral infection
a, 2 × 103TCF7-YFP reporter P14 CD8+ T cells (CD45.1−CD45.2+) were transferred into congenically distinct naïve mice (CD45.1+CD45.2−) prior to intravenous infection with 2 × 106 PFU of LCMV Armstrong (acute). On day 5, TCF7-YFP+Tim-3− (stem) andTCF7-YFP−Tim-3+ (effect.) P14 CD8+ T cells (CD45.1−CD45.2+) were FACS sorted from the spleen and 1 × 106 cells of each sorted subset were transferred intravenously into separate groups of congenically distinct, day 5 LCMV clone 13 (chronic) infected mice (CD45.1+CD45.2−). The recovery and phenotype of donor cells were analysed 8 days after transfer. b, Flow plots showing stem and effector subsets pre- and post-sort with purity indicated. c, FACs analysis showing frequencies of stem-like and effector P14 donor cells recovered from indicated tissues. Flow plots represent 1 independent experiment. d, Numbers of stem-like and effector P14 donor cells recovered from indicated tissues. Data are from 1 independent experiment with n = 3 mice (stem donor recipients) and n = 4 mice (effector donor recipients). e, Expression of indicated markers on stem-like donor P14 cells pre- (spleen, day 5 p.i., acute) and day 8 post-transfer (spleen, liver and lung, chronic). Arrow indicates differentiation trajectory of donor Tim-3− stem-like cells into Tim-3+ effector cells. f, Expression of indicated markers on effector donor P14 cells pre-transfer (spleen, day 5 p.i., acute, top panel) and day 8 post-transfer (spleen, chronic, bottom panel). Flow plots represent 1 independent experiment.
