Extended Data Fig. 5: Nociceptive neurons enhanced gastric ulcer regeneration and gastric cancer growth.

(a) Representative images of gastric ulcer samples and Ki-67 staining (n = 5 mice/group). Scale bar, 100 μm. Quantification of (b) ulcer area and (c) Ki-67 staining. (d) Representative MRI images from orthotopic GC model. (e) Representative images and (f) quantification of DT-induced nociceptive neuron ablation (n = 5 mice/group). Scale bar, 100 μm. (g) Representative images and (h) quantification of CGRP⁺ nerves in GCs from DT-treated mice (n = 10 mice/group). Scale bar, 100 μm. (i) Concentrations of CGRP peptide in GCs from DT-treated mice (n = 10 mice/group). (j) Representative images and (k) quantification of MNU-induced GCs from nociceptive neuron-ablated mice or control mice (n = 10 mice/group). (l) Representative images and (m) quantification of syngeneic orthotopic tumours from nociceptive neuron-ablated mice or control mice (n = 10 mice/group). (n) Representative images and (o) quantification of syngeneic orthotopic tumours from nociceptive neuron-ablated mice treated with vehicle or Rimegepant (n = 10 mice/group). (p) Representative images and (q) quantification of co-staining of mCherry and CGRP (n = 10 mice/group). Scale bar, 100 μm. (r) Representative images and (s) quantification of syngeneic orthotopic tumours from Calca-Cre mice with injection of AAV-hSyn-DIO-hM3Dq-mCherry (n = 10 mice/group). (t) Representative images and (u) quantification of syngeneic orthotopic tumours from C21-activated mice (n = 10 mice/group). Data represent mean ± SEM, and P values were calculated by t test in b, c, f, h, i, k, m, o, q, s and u. The statistical tests were two-sided.

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