Extended Data Fig. 2: Gastric epithelial injure upregulated Ngf expression.
From: Nociceptive neurons promote gastric tumour progression via a CGRP–RAMP1 axis
(a) Representative images of HE staining in mouse stomach treated with vehicle or DMP-777, and Ngf expression in epithelial cells (n = 5 mice/group). Scale bar, 100 μm. (b) Representative images of HE staining in mouse stomach treated with vehicle or high dose of MNU, and Ngf expression in epithelial cells (n = 5 mice/group). Scale bar, 100 μm. (c) Relative expression of Ngf in non-mutant (GFP−) and Kras-mutant (GFP+) gastric epithelial cells (n = 3 mice/group). Mist1+ Kras-mutant gastric epithelial cells were isolated from Mist1-CreERT; KrasG12D; tGFP mice after tamoxifen induction 1 month. (d) Relative expression of Ngf in total epithelial cells, YFP- epithelial cells and YFP+ malignant cells from Atp4b-Cre; Cdh1fl/fl; KrasG12D; Trp53 fl/fl; YFP mice (n = 5 mice/group). (e) Representative images of Ngf in situ hybridization in mouse GCs (n = 5 mice/group). Scale bar, 100 μm. (f) Representative images and (g) quantification of sensory nerves and micro vessels in NGF-overexpression mice and control mice (n = 5 mice/group). Scale bar, 100 μm. (h) Representative images of sensory nerves in mouse gastric cancers treated by Entrectinib or Vehicle (n = 5 mice/group). Scale bar, 100 μm. Data represent mean ± SEM, and P values were calculated by ANOVA in a, b and d, by t test in c and g. The statistical tests were two-sided.
