Extended Data Fig. 4: Process for creating, updating and releasing evolutionary models and PAN-GO (IBA) annotations derived from the models.

The central task is the software-assisted process of PAN-GO annotation and review using the PAINT tool. PAINT integrates the primary GO experimental annotations with the PANTHER trees built from UniProt Reference Proteomes (blue squares), allowing curators to construct an evolutionary model of each gene family, which is used to produce annotations in family members (green). Updates, both automated and curator-reviewed (orange squares), are made at each GO knowledgebase release to reflect changes in the underlying data (ontology and annotations), and upon release of new PANTHER versions.