Fig. 2: Developmental trajectories of POA cell types.
From: Sensory input, sex and function shape hypothalamic cell type development
a, Strategy for quantifying maturity as distance between each age’s centroid and the adult centroid in PCA space, calculated separately for each cell type. b, Distance trajectories across age for all cell types (grey), average (black) and extreme cell types of interest (coloured). a.u., arbitrary units. c, Top, schematic showing different trajectory classes. Bottom, distance trajectories across excitatory cell types. Trajectories were split into three classes depending on whether 90% of the maturation occurs across 4–5 ages (class 1, gradual), three ages (class 2, intermediate) or 1–2 ages (class 1, stepwise). d, Distance trajectories averaged across each class. e, Fractional class distribution of excitatory cell types, split by subregion. f, As in e but for inhibitory cell types. g, Fractional class distribution of all cell types or split by functional group. h,i, Distance trajectories for inhibitory VLPO (h) or MPN + VMPO + Pe (i) cell types. ***P < 0.0001 for two-way analysis of variance (ANOVA), effect of cell type subset. P = 2.15 × 10−11 (h) and 5.5 × 10−15 (i). j,k, Distance trajectories for cell types with roles in social behaviour (j) or homeostatic control (k). ***P < 0.0001 or NS (not significant) for two-way ANOVA, effect of cell type subset. P = 1.39 × 10−14 and 0.541. l, Eigentrend values summarize expression for devDEGs in select GO terms across age, for clusters of interest. Red, blue, and orange triangles indicate clusters referred to in the text as showing especially early (red, orange) or late (blue) changes.
