Fig. 5: Sensory signals affecting POA maturation.

a, Distance between mutant and control sample centroids in PCA space (normalized to intra-genotype distance) for excitatory clusters. Control samples included both C57BL/6J and control littermates. Sample numbers, left to right columns, mutant/control: 2/8, 4/10, 2/10, 2/8, 2/8, 2/8, 2/8, 2/7, 2/7, 2/8, 2/8, 2/7, 2/8 and 2/8. cKO, conditional knockout; KO, knockout. b, Mutant–control PCA distance for all clusters in TRPC2 P10 experiments, split according to functional role. c, Pseudotime mapping of Trpc2 mutant (−/−) and control (−/+ littermates) across age. Higher pseudotime values correspond to higher maturity (more similar to adult cell types). Pseudotime is averaged within a cluster; boxplot shows distribution across all cluster averages. P value indicates results from two-sided t-test, mutant versus control cell types. n for increasing age: 82, 137, 131 and 128 cell types for controls, and 119, 135, 129 and 108 cell types for mutants, from n for increasing age of 2, 4, 2 and 2 independent experiments per genotype. Boxplots in b and c show median, interquartile range and 1.5× interquartile range. d, Aggregated expression of 243 core neuronal maturation genes in wild-type C57BL/6J cell types at each age (left) or Trpc2^−/+ or Trpc2^−/− cell types at P10 (right). P value indicates results from two-sided t-test, mutant versus control cell types. e, Random-forest classifier approach (Augur) to identify cell types that show differences between Trpc2^−/+ and Trpc2^−/− cell types at P65. Each point represents a single cell type. Cell types with roles in relevant social behaviours affected in Trpc2^−/− adults are shown with coloured dots. AUC, area under the curve.