Extended Data Fig. 8: The effect of SNAr1.3 point mutations on the reaction rate with substrate 5. | Nature

Extended Data Fig. 8: The effect of SNAr1.3 point mutations on the reaction rate with substrate 5.

From: Engineered enzymes for enantioselective nucleophilic aromatic substitutions

Extended Data Fig. 8

Bar chart comparison of v0/[E] (min−1) values for point mutants of SNAr1.3 at residues that line the active site and halide binding cavity. Reaction conditions: 1 (75 mM), 5 (1.0 mM) and SNAr1.3 variant in NaPi pH 8.0 with 10% v/v DMSO at 30 °C. Enzyme concentrations: SNAr1.3 (1 μΜ); M64A, R65A, R88A, C96A (1 μΜ); R124A (25 μΜ); D125A (5 μΜ); D125N (40 μΜ). Error bars represent the standard deviation of measurements made in triplicate. See Supplementary Data for source data.

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