Extended Data Fig. 2: Kinetic characterization of SNAr1.0 and SNAr1.3.
From: Engineered enzymes for enantioselective nucleophilic aromatic substitutions
Michaelis–Menten plots for the SNAr reaction between 1 and 2 catalysed by either SNAr1.0 (a) or SNAr1.3 (b). Assays were performed at a fixed concentration of 1 (75 mM) and varying concentrations of 2, or a fixed concentration of 2 (2.5 mM) and varying concentrations of 1. The plots show the averaged initial rates that were fitted to the Michaelis–Menten equation using GraphPad Prism software. Error bars represent the standard deviation of measurements made in triplicate. See Supplementary Data for source data.
