Extended Data Fig. 4: Relationship between SNAr1.3 initial rate and concentration of chloride and iodide.
From: Engineered enzymes for enantioselective nucleophilic aromatic substitutions
Assays were performed at a fixed concentration of 1 (75 mM) and 5 (1 mM) with varying concentrations of either KCl (a) or KI (b). Linear fits of conversion versus time allowed determination of v0, and the v0 versus halide concentration steady-state kinetic data were fitted to the ‘[inhibitor] versus response’ equation (Y = Bottom + (Top − Bottom)/(1 + (X/IC50))) using GraphPad Prism, allowing for the calculation of IC50 values. Data points shown are averages of triplicate measurements, with error bars representing standard deviation. See Supplementary Data for source data.
