Extended Data Fig. 6: Changes in MAPP-identified peptides during bacterial infection.
From: Cell-autonomous innate immunity by proteasome-derived defence peptides
a, Bar graphs of IL-8 secretion from A549 cells infected with S. enterica at 1 or 4 h, or uninfected. mean ± s.e.m. (n = 3 biological replicates). One-way ANOVA, ** P < 0.0280. b, Heat map of correlation between biological sample replicates. c, PCA analysis comparing samples under different conditions. d-e, Volcano plots of degradome analysis with highlighted AMP-containing proteins at 1 h (d) and 4 h (e) of infection relative to uninfected control. f, Sequence alignment of UBE2M peptides identified by MAPP under different conditions: exclusive to control (light blue), infection (red), or shared (light gray). g, Absolute counts of peptides with cationic termini in control and infected samples. Data are mean ± s.e.m. (n = 3 biological replicates). One-way ANOVA, ** P < 0.01, **** P < 0.0001. h, Percentage of peptides with cationic or hydrophobic/aromatic C-terminal amino acids identified by MAPP in control or infected cells. i, Percentage of unique peptides with cationic or hydrophobic/aromatic N-terminal amino acids identified by MAPP exclusively in control or infected cells. j, Percentage of N-terminal amino acids of peptides identified by MAPP in control or infected. h-j, Two-sided Mann-Whitney U-test.
